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immunohistochemical staining for th  (Novus Biologicals)


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    Structured Review

    Novus Biologicals immunohistochemical staining for th
    Immunohistochemical Staining For Th, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/th+immunostaining/Tyrosine+Hydroxylase+Antibody/pmc12370004-100-11-19
    Average 96 stars, based on 268 article reviews
    immunohistochemical staining for th - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: Stereotactic body radiotherapy: a new non-invasive way to conduct pulmonary artery denervation
    Article Snippet: .. After the histological evaluation of all slides, sections from pulmonary arteries in each group were chosen for subsequent immunohistochemical staining against tyrosine hydroxylase (TH) (dilution 1:100, NB300-110SS, Novus). ..

    Article Title: Movement-related activity in the internal globus pallidus of the parkinsonian macaque
    Article Snippet: .. We have posted on protocols.io the step-by-step protocol used for the immunohistochemical staining for TH (protocol: https://dx.doi.org/10.17504/protocols.io.4r3l2q1dql1y/v1 ; antibody: Novus Cat# NB 300-109, RRID: AB_350437). ..

    Staining:

    Article Title: Stereotactic body radiotherapy: a new non-invasive way to conduct pulmonary artery denervation
    Article Snippet: .. After the histological evaluation of all slides, sections from pulmonary arteries in each group were chosen for subsequent immunohistochemical staining against tyrosine hydroxylase (TH) (dilution 1:100, NB300-110SS, Novus). ..

    Article Title: Movement-related activity in the internal globus pallidus of the parkinsonian macaque
    Article Snippet: .. We have posted on protocols.io the step-by-step protocol used for the immunohistochemical staining for TH (protocol: https://dx.doi.org/10.17504/protocols.io.4r3l2q1dql1y/v1 ; antibody: Novus Cat# NB 300-109, RRID: AB_350437). ..

    other:

    Article Title: Ferroptosis in the Substantia Nigra Pars Compacta of Mice: Triggering Role of Ultrafine Diesel Exhaust Particles and Mitigation by α-Lipoic Acid.
    Article Snippet: 1 Department of Anatomy, Chungbuk National University College of Medicine, Chungdae-ro 1, Seowon-gu, Cheongju, Chungbuk 28644, Republic of Korea 2 Department of Neurology, Chungbuk National University Hospital, Chungbuk National University College of Medicine, Cheongju, Chungbuk 28644, Republic of Korea 3 Department of Biomedical Laboratory Science, College of Health Science, Cheongju University, Cheongju, Chungbuk 28503, Republic of Korea 4 Department of Preventive Medicine, Chungbuk National University College of Medicine, Cheongju, Chungbuk 28644, Republic of Korea 5 Biomedical Research Institute, Chungbuk National University Hospital, Cheongju, Chungbuk 28644, Republic of Korea 6 Chungbuk Regional Cancer Center, Chungbuk National University Hospital, Cheongju, Chungbuk 28644, Republic of Korea 7 Chungbuk Environmental Health Center, Chungbuk National University Hospital, Cheongju, Chungbuk 28644, Republic of Korea Abstract Recent epidemiological and experimental studies have increasingly highlighted the association between environmental pollution, especially ultrafine particulate matter (PM), and the risk of neurodegenerative diseases, such as Parkinson’s disease (PD).. These previous studies suggest a potential mechanism by which ultrafine PM contributes to neuronal damage through processes, such as iron accumulation and oxidative stress.. In this study, we aimed to elucidate the effects of ultrafine PM on ferroptosis, an iron-dependent form of cell death, in the mouse substantia nigra pars compacta (SNc) and to evaluate the protective role of α-lipoic acid (ALA).

    Article Title: Deficiency of Cullin 3, a Protein Encoded by a Schizophrenia and Autism Risk Gene, Impairs Behaviors by Enhancing the Excitability of Ventral Tegmental Area (VTA) DA Neurons
    Article Snippet: Primary antibodies used were as follows: Phospho-Akt (Thr308) (1:1000 for Western blot; 9275, Cell Signaling Technology); Akt (1:1000 for Western blot; 9272, Cell Signaling Technology); Phospho-TH (Ser40) (1:1000 for Western blot; 2791, Cell Signaling Technology); TH (1:1000 for Western blot, 1:1000 for immunostaining; NB300-109, Novus Biologicals); D2DR (1:250 for Western blot; ADR-002, Alomone Labs); Cullin3 (1:500 for Western blot; A301-109A, Bethyl Laboratories); b -actin (1:5000 for Western blot; ab8226, Abcam); NeuN (1:500 for immunostaining; MAB377, Millipore); HCN1 (1:1000 for Western blot; PA5-78 675, Invitrogen); HCN2 (1:500 for Western blot; PA5-77594, Invitrogen); HCN3 (1:1000 for Western blot; PA5-104434, Invitrogen); HCN4 (1:250 for Western blot; PA5-111878, Invitrogen); Poly-Ubiquitin (1:1000 for Western blot; K63 Linkage, NBP2-36523, Novus Biologicals); and RFP (1:1000 for immunostaining; 600-401-379, Rockland).

    Article Title: ATPase Thorase Deficiency Causes α-Synucleinopathy and Parkinson's Disease-like Behavior.
    Article Snippet: Scale bars, 1 mm for low magnification and 50 μm for high magnification. (H) Quantification of TH+ fibers in the STR from Thorase cKO-A53T and A53T littermate mice (n = 4). (I) Representative images of TH immunostaining using anti-TH antibody (Novus, 1:1000) in SNpc regions from Thorase cKO-A53T and A53T littermate mice at the age of 5 months.

    Incubation:

    Article Title: Inhibition of de novo ceramide synthesis mitigates alpha-synuclein pathology in a Parkinson’s disease mouse model
    Article Snippet: Sections were washed in PBS containing 1% Tween-20 (PBS-T) and incubated with BLOXALL® Endogenous Blocking Solution (Vector Laboratories, SP-6000) for 20 min to quench endogenous peroxidase activity. .. After washing, sections were blocked with 2% normal serum in PBS-T for 2 h at room temperature, followed by overnight incubation at 4 °C with primary antibody against tyrosine hydroxylase (TH; Novus, OPA1-04050, 1:1000). .. The next day, sections were washed in PBS-T and incubated with a biotinylated anti-rabbit secondary antibody included in the Alkaline Phosphatase Standard Kit (Vector Laboratories, AK-5200) for 30 min, followed by alkaline phosphatase reagent (Vector Laboratories, AK-5200) for an additional 30 min. Signal was developed using Vector® Red Substrate Kit (Vector Laboratories, SK-5100).

    Immunostaining:

    Article Title: β-adrenergic signaling blockade attenuates metastasis through activation of cytotoxic CD4 T cells
    Article Snippet: For Ki67 immunostaining, Target Retrieval Solution, Citrate pH 6.1 (S1699, Agilent), rabbit anti-mouse Ki67 (1:100, AB16667, clone SP6, Abcam) were used. .. For TH immunostaining, Target Retrieval Solution, Citrate pH 6.1 (S1699, Agilent), polyclonal rabbit anti-mouse TH (1:500, NB300-109, Novus Biologicals) were used. .. The sections were then stained with EnVision rabbit (K4003, Agilent) with NOVAred substrate (SK4800, Vector).



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    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) <t>Immunohistochemical</t> staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine <t>hydroxylase;</t> Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.
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    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) <t>Immunohistochemical</t> staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine <t>hydroxylase;</t> Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.
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    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) <t>Immunohistochemical</t> staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine <t>hydroxylase;</t> Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.
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    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) <t>Immunohistochemical</t> staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine <t>hydroxylase;</t> Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.
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    Image Search Results


    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) Immunohistochemical staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine hydroxylase; Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.

    Journal: Frontiers in Medicine

    Article Title: Stereotactic body radiotherapy: a new non-invasive way to conduct pulmonary artery denervation

    doi: 10.3389/fmed.2025.1607638

    Figure Lengend Snippet: Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) Immunohistochemical staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine hydroxylase; Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.

    Article Snippet: After the histological evaluation of all slides, sections from pulmonary arteries in each group were chosen for subsequent immunohistochemical staining against tyrosine hydroxylase (TH) (dilution 1:100, NB300-110SS, Novus).

    Techniques: Staining, Control, Immunohistochemical staining, MANN-WHITNEY